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KMID : 0900919930170030193
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Korean journal of Animal Reproduction
1993 Volume.17 No. 3 p.193 ~ p.199
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The Effect of Pronuclear Injection of Recombinant DNA on the Development Potential of Bovine Follicular Oocytes In Vitro
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Lee Chul-Sang
Han Yong-Mahn
Yu Dae-Yeul
Lee Kyung-Kwang
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Abstract
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Bovine follicular oocytes were matured in two different conditions, TCM 199＋10% FBS with or without hormones (0.01 unit/ml ovine follicle stimulating hormone, 0.01 unit/ml ovine luteinizing hormone and 1mutextrm{g}/ml beta-estradiol). There was no significant difference in maturation and fertilization rates of the oocytes between two groups. The result indicates that hormonal treatment does not have beneficial effect on in vitro maturation and fertilization of follicular oocytes. IVF-derived cone-cell bovine embryos were injected with foreign DNA (CChcLf) by microinjection method and then co-cultrued with bovine oviductal epithelial cells. Developmental rate of microinjected embryos to blastocyst stage (21%) was similar to that of non-injected embryos(29%). This result represents that microinjected bovine embryos produced in vitro have a potential of development to normal blastocysts.
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KEYWORD
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